软件安装 BUSCO的手动安装并不困难，但是BUSCO依赖tblastn与augustus，而augustus的安装比较复杂，因此选择使用co

install 1 2 3 wget https://github.com/mahulchak/quickmerge/archive/v0.2.tar.gz tar zxf v0.2.tar.gz bash make_merger.sh Running The simplest way to run ‘merger’ is to use the python wrapper ‘merge_wrapper_v2.py’: 1 merge_wrapper.py hybrid_assembly.fasta self_assembly.fasta Which of my assemblies should I use as query and reference? There is no golden rule for this. Quickmerge was developed with the idea that every genome is different and that you know best about the genome you are assembling. All

In the README of quickmerge, I got a advice. Assembly polishing with Quiver and pilon before and after assembly merging is strongly recommended. As for Sequal data, Pacbio updated the quiver to arrow. Quiver is supported for PacBio RS data. Arrow is supported for PacBio Sequel data and RS data with the P6-C4 chemistry. So, let’s do it ! Step 0 Install SMRT Tools 1 2 SMRT_ROOT=/opt/pacbio/smrtlink .

安装Perlberw 1 \curl -L https://install.perlbrew.pl | bash 安装完成后即可不需sudo安装模块，尝试不同版本的perl 使用cpanminus管理模块 使用perlberw

建立数据库 1 ${RepeatModelerPath}/BuildDatabase -name ${database_name} ${fasta} 但是对于ABBlast来说，BuildDatabase建立的索引还缺少nt_db.xni文件，出现提示 1 2 3 4 FATAL: xdf_db_fopen failed code 22